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dopamine receptor d2 (d2r) cytoplasmic loop #3 (28 aa) of human d2r  (Millipore)


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    Structured Review

    Millipore dopamine receptor d2 (d2r) cytoplasmic loop #3 (28 aa) of human d2r
    Information on primary antibodies.
    Dopamine Receptor D2 (D2r) Cytoplasmic Loop #3 (28 Aa) Of Human D2r, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dopamine+d2+receptors+(d2r/quinpirole++a+d2+like+receptor+agonist+/pmc09971002-1-11-14
    Average 90 stars, based on 1 article reviews
    dopamine receptor d2 (d2r) cytoplasmic loop #3 (28 aa) of human d2r - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex"

    Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

    Journal: Frontiers in Neuroanatomy

    doi: 10.3389/fnana.2023.1111008

    Information on primary antibodies.
    Figure Legend Snippet: Information on primary antibodies.

    Techniques Used: Purification

    Distribution of each dopamine receptor in the motor cortex and caudate putamen. (A) Representative immunohistochemistry of the frontal cortex for D1R. High-magnification images of rectangles indicated with E and I are shown in (E,I) . Moderate to strong D1R-immunoreactivities were observed in the pial surface of the cerebral cortex, layer (VI), and caudate putamen. (B) Representative immunohistochemistry of the frontal cortex for D2R. High magnification images of rectangles indicated with F and J are shown in (F) and (J) . (C) Representative immunohistochemistry of the frontal cortex for D4R. High magnification images of rectangles indicated with G and K are shown in (G) and (K) . (D) Representative immunohistochemistry of the frontal cortex for D5R. High magnification images of rectangles indicated with H and L are shown in (H) and (L) . (E–H) Immunohistochemical images of the cortical surface for D1R (E) , D2R (F) , D4R (G) , and D5R (H) . (I) Immunohistochemical images of the cortical layer V for D1R. Neuronal somata show weak immunopositive signals for D1R (arrowheads). (J) Immunohistochemical images of the cortical layer V for D2R. Neuronal somata show weak immunoreactivity for D2R (arrowheads). (K,L) Immunohistochemical images of the cortical layer V for D4R or D5R. Many neuronal somata (arrowheads) and apical dendrites (arrows) show immunoreactivity for D4R or D5R. Scale bars: 500 μm (A–D) ; 125 μm (E–H) ; 50 μm (I–L) .
    Figure Legend Snippet: Distribution of each dopamine receptor in the motor cortex and caudate putamen. (A) Representative immunohistochemistry of the frontal cortex for D1R. High-magnification images of rectangles indicated with E and I are shown in (E,I) . Moderate to strong D1R-immunoreactivities were observed in the pial surface of the cerebral cortex, layer (VI), and caudate putamen. (B) Representative immunohistochemistry of the frontal cortex for D2R. High magnification images of rectangles indicated with F and J are shown in (F) and (J) . (C) Representative immunohistochemistry of the frontal cortex for D4R. High magnification images of rectangles indicated with G and K are shown in (G) and (K) . (D) Representative immunohistochemistry of the frontal cortex for D5R. High magnification images of rectangles indicated with H and L are shown in (H) and (L) . (E–H) Immunohistochemical images of the cortical surface for D1R (E) , D2R (F) , D4R (G) , and D5R (H) . (I) Immunohistochemical images of the cortical layer V for D1R. Neuronal somata show weak immunopositive signals for D1R (arrowheads). (J) Immunohistochemical images of the cortical layer V for D2R. Neuronal somata show weak immunoreactivity for D2R (arrowheads). (K,L) Immunohistochemical images of the cortical layer V for D4R or D5R. Many neuronal somata (arrowheads) and apical dendrites (arrows) show immunoreactivity for D4R or D5R. Scale bars: 500 μm (A–D) ; 125 μm (E–H) ; 50 μm (I–L) .

    Techniques Used: Immunohistochemistry, Immunohistochemical staining

    D2R-immunoreactivity in astrocytes. (A,B) Very weak immunoreactivities for D2R were observed in the pial surface of the prelimbic (A) and somatosensory (B) areas. (C,D) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated a few weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the prelimbic area. (E,F) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the primary motor area. (G,H) Weak D2R-immunoreactivities were observed in layer I astrocytes (arrowheads) in the somatosensory area. (I,J) In layer III of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (arrowheads), but not in GS-positive protoplasmic astrocytes (arrows). (K,L) In layer V of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (asterisk), but only very weak in GS-positive protoplasmic astrocytes (arrows). Nuclei were stained with Hoechst33342. Scale bars: 20 μm (A,B) ; 5 μm (C,D) , 10 μm (E–L) .
    Figure Legend Snippet: D2R-immunoreactivity in astrocytes. (A,B) Very weak immunoreactivities for D2R were observed in the pial surface of the prelimbic (A) and somatosensory (B) areas. (C,D) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated a few weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the prelimbic area. (E,F) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the primary motor area. (G,H) Weak D2R-immunoreactivities were observed in layer I astrocytes (arrowheads) in the somatosensory area. (I,J) In layer III of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (arrowheads), but not in GS-positive protoplasmic astrocytes (arrows). (K,L) In layer V of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (asterisk), but only very weak in GS-positive protoplasmic astrocytes (arrows). Nuclei were stained with Hoechst33342. Scale bars: 20 μm (A,B) ; 5 μm (C,D) , 10 μm (E–L) .

    Techniques Used: Imaging, Double Immunostaining, Staining

    Related Articles

    Polyacrylamide Gel Electrophoresis:

    Article Title: MeCP2 in the Rostral Striatum Maintains Local Dopamine Content Critical for Psychomotor Control
    Article Snippet: Brain tissues were homogenized by sonication in lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Sigma-Aldrich). .. Twenty micrograms of protein was separated by PAGE (10%, Bio-Rad) with 60 V for 30 min and 120 V for 1.5 h and then transferred to a PVDF membrane (Millipore) by liquid electroblotting (Mini Trans-Blot Cell, Bio-Rad) with 350 mA for 1 h. The membrane was blocked by skim milk and incubated with primary antibodies against MeCP2 (1:2000; Zhou et al., 2006 ), dopamine D2 receptor (DRD2, 1:1000, Millipore), tyrosine hydroxylase (TH; 1:20,000, Millipore), phosphor-TH at Ser40 (pTH-Ser40, 1:2000, Symansis), or glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:100,000, Millipore) at 4°C for 16 h. Following incubation with peroxidase-conjugated goat-anti-rabbit or goat-anti-mouse secondary antibodies (1:100,000) at room temperature for 2 h, the expression of the interested proteins was detected by an enhanced chemoluminescence reagent kit (Millipore) under a bioimage acquisition system (Xlite 200R, Avegene Life Science). ..

    Article Title: MeCP2 in the Rostral Striatum Maintains Local Dopamine Content Critical for Psychomotor Control
    Article Snippet: Brain tissues were homogenized by sonication in lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Sigma-Aldrich). .. Twenty micrograms of protein was separated by PAGE (10%, Bio-Rad) with 60 V for 30 min and 120 V for 1.5 h and then transferred to a PVDF membrane (Millipore) by liquid electroblotting (Mini Trans-Blot Cell, Bio-Rad) with 350 mA for 1 h. The membrane was blocked by skim milk and incubated with primary antibodies against MeCP2 (1:2000; Zhou et al., 2006), dopamine D2 receptor (DRD2, 1:1000, Millipore), tyrosine hydroxylase (TH; 1:20,000, Millipore), phosphor-TH at Ser40 (pTH-Ser40, 1:2000, Symansis), or glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:100,000, Millipore) at 4°C for 16 h. Following incubation with peroxidase-conjugated goat-antirabbit or goat-anti-mouse secondary antibodies (1:100,000) at room temperature for 2 h, the expression of the interested proteins was detected by an enhanced chemoluminescence reagent kit (Millipore) under a bioimage acquisition system (Xlite 200R, Avegene Life Science). ..

    Membrane:

    Article Title: MeCP2 in the Rostral Striatum Maintains Local Dopamine Content Critical for Psychomotor Control
    Article Snippet: Brain tissues were homogenized by sonication in lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Sigma-Aldrich). .. Twenty micrograms of protein was separated by PAGE (10%, Bio-Rad) with 60 V for 30 min and 120 V for 1.5 h and then transferred to a PVDF membrane (Millipore) by liquid electroblotting (Mini Trans-Blot Cell, Bio-Rad) with 350 mA for 1 h. The membrane was blocked by skim milk and incubated with primary antibodies against MeCP2 (1:2000; Zhou et al., 2006 ), dopamine D2 receptor (DRD2, 1:1000, Millipore), tyrosine hydroxylase (TH; 1:20,000, Millipore), phosphor-TH at Ser40 (pTH-Ser40, 1:2000, Symansis), or glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:100,000, Millipore) at 4°C for 16 h. Following incubation with peroxidase-conjugated goat-anti-rabbit or goat-anti-mouse secondary antibodies (1:100,000) at room temperature for 2 h, the expression of the interested proteins was detected by an enhanced chemoluminescence reagent kit (Millipore) under a bioimage acquisition system (Xlite 200R, Avegene Life Science). ..

    Article Title: MeCP2 in the Rostral Striatum Maintains Local Dopamine Content Critical for Psychomotor Control
    Article Snippet: Brain tissues were homogenized by sonication in lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Sigma-Aldrich). .. Twenty micrograms of protein was separated by PAGE (10%, Bio-Rad) with 60 V for 30 min and 120 V for 1.5 h and then transferred to a PVDF membrane (Millipore) by liquid electroblotting (Mini Trans-Blot Cell, Bio-Rad) with 350 mA for 1 h. The membrane was blocked by skim milk and incubated with primary antibodies against MeCP2 (1:2000; Zhou et al., 2006), dopamine D2 receptor (DRD2, 1:1000, Millipore), tyrosine hydroxylase (TH; 1:20,000, Millipore), phosphor-TH at Ser40 (pTH-Ser40, 1:2000, Symansis), or glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:100,000, Millipore) at 4°C for 16 h. Following incubation with peroxidase-conjugated goat-antirabbit or goat-anti-mouse secondary antibodies (1:100,000) at room temperature for 2 h, the expression of the interested proteins was detected by an enhanced chemoluminescence reagent kit (Millipore) under a bioimage acquisition system (Xlite 200R, Avegene Life Science). ..

    Incubation:

    Article Title: MeCP2 in the Rostral Striatum Maintains Local Dopamine Content Critical for Psychomotor Control
    Article Snippet: Brain tissues were homogenized by sonication in lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Sigma-Aldrich). .. Twenty micrograms of protein was separated by PAGE (10%, Bio-Rad) with 60 V for 30 min and 120 V for 1.5 h and then transferred to a PVDF membrane (Millipore) by liquid electroblotting (Mini Trans-Blot Cell, Bio-Rad) with 350 mA for 1 h. The membrane was blocked by skim milk and incubated with primary antibodies against MeCP2 (1:2000; Zhou et al., 2006 ), dopamine D2 receptor (DRD2, 1:1000, Millipore), tyrosine hydroxylase (TH; 1:20,000, Millipore), phosphor-TH at Ser40 (pTH-Ser40, 1:2000, Symansis), or glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:100,000, Millipore) at 4°C for 16 h. Following incubation with peroxidase-conjugated goat-anti-rabbit or goat-anti-mouse secondary antibodies (1:100,000) at room temperature for 2 h, the expression of the interested proteins was detected by an enhanced chemoluminescence reagent kit (Millipore) under a bioimage acquisition system (Xlite 200R, Avegene Life Science). ..

    Article Title: MeCP2 in the Rostral Striatum Maintains Local Dopamine Content Critical for Psychomotor Control
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    Article Title: Transient Chemogenetic Inhibition of D1-MSNs in the Dorsal Striatum Enhances Methamphetamine Self-Administration
    Article Snippet: Each sample containing protein from one animal was run (20 μg per lane) on 8% SDS-PAGE gels (Bio-Rad) and transferred to polyvinylidene fluoride membranes (PVDF pore size 0.2 μm). .. Blots were blocked with 2.5% bovine serum albumin (for phosphoproteins) or 5% milk (w/v) in TBST (25 mM Tris–HCl (pH 7.4), 150 mM NaCl and 0.1% Tween 20 (v/v)) for 16–20 h at 4 °C and were incubated with the primary antibody for 16–20 h at 4 °C: antibody to phosphorylated-p44/42 MAPK (pErk1/2) at Thr202/Tyr204 (mouse monoclonal, 1:1000, Cell Signaling cat# 9106S, molecular weights 44/42 kDa); total Erk1/2 (rabbit polyclonal, 1:4000, Cell Signaling cat# 9102, molecular weights 44/42 kDa); pCamKII Tyr-286 (rabbit polyclonal, 1:1000, Abcam cat# ab5683, molecular weight 50 kDa); total CaMKII (rabbit polyclonal, 1:2000, Abcam cat# ab52476, molecular weight 47 kDa); pAkt Ser-473 (rabbit polyclonal, 1:1000, Cell Signaling cat# 4060S, molecular weight 60 kDa); total Akt (rabbit polyclonal, 1:1000, Cell Signaling cat# 4691S, molecular weight 60 kDa); D1R (rabbit polyclonal, 1:1000, Abcam cat# ab20066, molecular weight 48 kDa); Cav-1 (rabbit monoclonal, 1:1000, Cell Signaling cat# 3267S, molecular weight 24 kDa); PSD-95 (rabbit polyclonal, 1:500, Millipore cat# 04-1066, molecular weight 95 kDa); Dopamine D2 receptors (D2R, rabbit polyclonal, 1:1000, Sigma Aldrich cat# AB5084P, molecular weight 48 kDa); dopamine transporter (DAT; rat monoclonal, 1:500, Santacruz cat# sc-32258, molecular weight 90 kDa). .. Blots were then washed with TBS-T and incubated for 1h at room temperature with horseradish peroxide-conjugated goat antibody to rabbit or horseradish peroxide–conjugated goat antibody to mouse (1:5,000, BioRad) in TBS-T.

    Expressing:

    Article Title: MeCP2 in the Rostral Striatum Maintains Local Dopamine Content Critical for Psychomotor Control
    Article Snippet: Brain tissues were homogenized by sonication in lysis buffer containing 1% protease inhibitors and phosphatase inhibitors (Sigma-Aldrich). .. Twenty micrograms of protein was separated by PAGE (10%, Bio-Rad) with 60 V for 30 min and 120 V for 1.5 h and then transferred to a PVDF membrane (Millipore) by liquid electroblotting (Mini Trans-Blot Cell, Bio-Rad) with 350 mA for 1 h. The membrane was blocked by skim milk and incubated with primary antibodies against MeCP2 (1:2000; Zhou et al., 2006 ), dopamine D2 receptor (DRD2, 1:1000, Millipore), tyrosine hydroxylase (TH; 1:20,000, Millipore), phosphor-TH at Ser40 (pTH-Ser40, 1:2000, Symansis), or glyceraldehyde 3-phosphate dehydrogenase (GAPDH; 1:100,000, Millipore) at 4°C for 16 h. Following incubation with peroxidase-conjugated goat-anti-rabbit or goat-anti-mouse secondary antibodies (1:100,000) at room temperature for 2 h, the expression of the interested proteins was detected by an enhanced chemoluminescence reagent kit (Millipore) under a bioimage acquisition system (Xlite 200R, Avegene Life Science). ..

    Article Title: MeCP2 in the Rostral Striatum Maintains Local Dopamine Content Critical for Psychomotor Control
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    other:

    Article Title: Reducing Glypican-4 in ES Cells Improves Recovery in a Rat Model of Parkinson's Disease by Increasing the Production of Dopaminergic Neurons and Decreasing Teratoma Formation
    Article Snippet: Antibodies included the following: anti-TH (Aves, 1:1000; or Immunostar 1:1600), anti-GFP (Aves; 1:500), anti-nestin (Aves; 1:1000), anti-DAT (Santa Cruz Biotechnology; 1:100), anti-Pitx3 (gift from Austin Smith, 1:1000), anti-FoxA2 (Santa Cruz Biotechnology; 1:100), anti-calbindin (Millipore; 1:1000), anti-GABA (Sigma; 1:300), anti-serotonin (5-HT) (Immunostar; 1:500), and anti- dopamine D2 receptor (DRD2) (Millipore/Abcam; 1:150; specificity of the antibodies was validated on sections at the level of the substantia nigra).

    Article Title: Adolescent stress leads to glutamatergic disturbance through dopaminergic abnormalities in the prefrontal cortex of genetically vulnerable mice
    Article Snippet: Rabbit anti-phospho- NMDA receptor subunit 1 (p-NR1; 1:500, Millipore), goat anti-total-NR1 (t-NR1; 1:500, Santa Cruz Biotechnology), rabbit anti- phospho-CaMK II (p-CaMK II; 1:1000, Millipore), rabbit anti-total-CaMK II (t-CaMK II; 1:1000, Sigma-Aldrich), guinea-pig anti-GLAST (1:1000, Millipore), guinea-pig anti-GLT-1 (1:1000, Millipore), mouse anti-tyrosine hydroxylase (TH; 1:1000, Millipore), rabbit anti- dopamine D2 receptor (D2R; 1:500, Millipore), and goat anti-β-actin (1:500, Santa Cruz Biotechnology) antibodies were used as primary antibodies.

    Article Title: Orexin-A/Hypocretin-1 Controls the VTA-NAc Mesolimbic Pathway via Endocannabinoid-Mediated Disinhibition of Dopaminergic Neurons in Obese Mice
    Article Snippet: Alternate brain slices (10 μm of thickness) containing dorsal or ventral striatum of mice were collected in three series of slides and then processed for multiple immunofluorescence by overnight incubation in a mixture of the following primary antibodies, each diluted 1:100 in PB-T: (1) goat anti-OX-A (Santa Cruz Biotechnology, Inc., Dallas, Texas–USA) and mouse anti-TH (Millipore); (2) mouse anti-TH (Millipore) and rabbit anti-Neuronal Marker (anti-NeuN) (Abcam); (3) goat anti-OX1R (Santa Cruz Biotechnology), guinea pig anti-diacylglycerol lipase-α (anti-DAGL-α) (kindly provided by Prof. Ken Mackie) and mouse anti-TH (Millipore); (4) goat anti-OX-A (Santa Cruz Biotechnology), guinea pig anti-vesicular glutamate transporter (anti-VGluT1) (Synaptic Systems) and mouse anti-TH (Millipore); (5) rabbit anti-CB1R antibody (anti C terminus 461-472, Abcam), guinea pig anti-vesicular GABA transporter (anti-VGAT) (Synaptic Systems) and mouse anti-TH (Millipore); (6) rabbit anti-dopamine D2 Receptor (anti-D2R) (Millipore) and goat anti-βarrestin2 (Santa Cruz Biotechnology); (7) goat anti c- Fos (Santa Cruz Biotechnology) and rabbit anti-D2R (Millipore).

    Article Title: Autologous transplantation of GDNF-expressing mesenchymal stem cells protects against MPTP-induced damage in cynomolgus monkeys
    Article Snippet: Antibodies used were mouse anti-monkey tyrosine hydroxylase (TH) (1:500, Chemicon), mouse anti-human glial fibrillary acidic protein (GFAP) polyclonal antibody (1:1000, Chemicon), goat anti-human Iba1 polyclonal antibody (1:100, abcam), mouse anti-monkey choline acetyltransferase monoclonal antibody (ChAT) (1:500, abcam), rat anti-human dopamine receptor D1 monoclonal antibody (D1R) (1:200, Sigma-aldrich), rabbit anti-human dopamine receptor D2 (L/S) polyclonal antibody (D2R) (1:1000, Chemicon).

    Article Title: Prenatal Immune Activation Interacts with Genetic Nurr1 Deficiency in the Development of Attentional Impairments
    Article Snippet: The following primary antibodies were used to study dopamine-related markers in various brain areas according to protocols established previously (Meyer et al., 2008a; Bitanihirwe et al., 2010a; Vuillermot et al., 2010): rabbit anti-TH (Santa Cruz Biotechnology; diluted 1:500), rabbit antidopamine D1 receptor (D1R) (Millipore Bioscience Research Reagents; diluted 1:250), rabbit anti-dopamine D2 receptor (D2R) (Millipore Bioscience Research Reagents; diluted 1:500), and rabbit anti-catechol-Omethyltransferase (COMT) (SDI; diluted 1:5000).

    Molecular Weight:

    Article Title: Transient Chemogenetic Inhibition of D1-MSNs in the Dorsal Striatum Enhances Methamphetamine Self-Administration
    Article Snippet: Each sample containing protein from one animal was run (20 μg per lane) on 8% SDS-PAGE gels (Bio-Rad) and transferred to polyvinylidene fluoride membranes (PVDF pore size 0.2 μm). .. Blots were blocked with 2.5% bovine serum albumin (for phosphoproteins) or 5% milk (w/v) in TBST (25 mM Tris–HCl (pH 7.4), 150 mM NaCl and 0.1% Tween 20 (v/v)) for 16–20 h at 4 °C and were incubated with the primary antibody for 16–20 h at 4 °C: antibody to phosphorylated-p44/42 MAPK (pErk1/2) at Thr202/Tyr204 (mouse monoclonal, 1:1000, Cell Signaling cat# 9106S, molecular weights 44/42 kDa); total Erk1/2 (rabbit polyclonal, 1:4000, Cell Signaling cat# 9102, molecular weights 44/42 kDa); pCamKII Tyr-286 (rabbit polyclonal, 1:1000, Abcam cat# ab5683, molecular weight 50 kDa); total CaMKII (rabbit polyclonal, 1:2000, Abcam cat# ab52476, molecular weight 47 kDa); pAkt Ser-473 (rabbit polyclonal, 1:1000, Cell Signaling cat# 4060S, molecular weight 60 kDa); total Akt (rabbit polyclonal, 1:1000, Cell Signaling cat# 4691S, molecular weight 60 kDa); D1R (rabbit polyclonal, 1:1000, Abcam cat# ab20066, molecular weight 48 kDa); Cav-1 (rabbit monoclonal, 1:1000, Cell Signaling cat# 3267S, molecular weight 24 kDa); PSD-95 (rabbit polyclonal, 1:500, Millipore cat# 04-1066, molecular weight 95 kDa); Dopamine D2 receptors (D2R, rabbit polyclonal, 1:1000, Sigma Aldrich cat# AB5084P, molecular weight 48 kDa); dopamine transporter (DAT; rat monoclonal, 1:500, Santacruz cat# sc-32258, molecular weight 90 kDa). .. Blots were then washed with TBS-T and incubated for 1h at room temperature with horseradish peroxide-conjugated goat antibody to rabbit or horseradish peroxide–conjugated goat antibody to mouse (1:5,000, BioRad) in TBS-T.



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    Information on primary antibodies.
    Dopamine Receptor D2 (D2r) Cytoplasmic Loop #3 (28 Aa) Of Human D2r, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dopamine+d2+receptors+(d2r/quinpirole++a+d2+like+receptor+agonist+/pmc09971002-1-11-14
    Average 90 stars, based on 1 article reviews
    dopamine receptor d2 (d2r) cytoplasmic loop #3 (28 aa) of human d2r - by Bioz Stars, 2026-10
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    Millipore dopamine receptor d2 cytoplasmic loop #3 (28 aa) of human d2r antibody
    Information on primary antibodies.
    Dopamine Receptor D2 Cytoplasmic Loop #3 (28 Aa) Of Human D2r Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dopamine+d2+receptors+(d2r/chilled+ultrafiltration+loop+3+m2+of+millipore+plchk+membranes/pm36865631-72-32-34
    Average 90 stars, based on 1 article reviews
    dopamine receptor d2 cytoplasmic loop #3 (28 aa) of human d2r antibody - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


     Dopamine receptor subtype  agonists and antagonists used in this study.

    Journal: PLOS ONE

    Article Title: Characterization of the atypical antipsychotic drug aripiprazole cytotoxicity in the neutrophil model cell line HL-60

    doi: 10.1371/journal.pone.0318878

    Figure Lengend Snippet: Dopamine receptor subtype agonists and antagonists used in this study.

    Article Snippet: The following primary antibodies were used to detect specific dopamine receptor subtypes: PE mouse anti-human Dopamine D1 receptor (BioLegend), rabbit anti-dopamine D2R (extracellular) receptor (Alomone labs), rabbit anti-dopamine D3R receptor (MilliporeSigma), rabbit anti-dopamine D4R receptor (Invitrogen), and rabbit anti-dopamine D5R receptor (Invitrogen).

    Techniques:

    Flow cytometry analysis of dopamine receptor surface expression on dHL-60 cells using polyclonal antibodies directed against extracellular epitopes of specific dopamine receptor subtypes. Representative histograms from three independent replicates illustrate the fluorescence intensity of unstained negative controls (black line), secondary antibody controls (blue line), IgG isotype controls (green line), and dopamine-specific receptors (red line).

    Journal: PLOS ONE

    Article Title: Characterization of the atypical antipsychotic drug aripiprazole cytotoxicity in the neutrophil model cell line HL-60

    doi: 10.1371/journal.pone.0318878

    Figure Lengend Snippet: Flow cytometry analysis of dopamine receptor surface expression on dHL-60 cells using polyclonal antibodies directed against extracellular epitopes of specific dopamine receptor subtypes. Representative histograms from three independent replicates illustrate the fluorescence intensity of unstained negative controls (black line), secondary antibody controls (blue line), IgG isotype controls (green line), and dopamine-specific receptors (red line).

    Article Snippet: The following primary antibodies were used to detect specific dopamine receptor subtypes: PE mouse anti-human Dopamine D1 receptor (BioLegend), rabbit anti-dopamine D2R (extracellular) receptor (Alomone labs), rabbit anti-dopamine D3R receptor (MilliporeSigma), rabbit anti-dopamine D4R receptor (Invitrogen), and rabbit anti-dopamine D5R receptor (Invitrogen).

    Techniques: Flow Cytometry, Analysis, Expressing, Fluorescence, FACS

    dHL-60 cells were treated with 20 μM of the dopamine receptor antagonist olanzapine, flupentixol, or eticlopride in the absence or presence of aripiprazole for 48 hours in media containing 2% serum. The average absorbance relative to DMSO control was graphed from at least three replicates. Error bars indicate the standard error of the mean. * indicates statistical significance via ANOVA with Tukey’s post-hoc test.

    Journal: PLOS ONE

    Article Title: Characterization of the atypical antipsychotic drug aripiprazole cytotoxicity in the neutrophil model cell line HL-60

    doi: 10.1371/journal.pone.0318878

    Figure Lengend Snippet: dHL-60 cells were treated with 20 μM of the dopamine receptor antagonist olanzapine, flupentixol, or eticlopride in the absence or presence of aripiprazole for 48 hours in media containing 2% serum. The average absorbance relative to DMSO control was graphed from at least three replicates. Error bars indicate the standard error of the mean. * indicates statistical significance via ANOVA with Tukey’s post-hoc test.

    Article Snippet: The following primary antibodies were used to detect specific dopamine receptor subtypes: PE mouse anti-human Dopamine D1 receptor (BioLegend), rabbit anti-dopamine D2R (extracellular) receptor (Alomone labs), rabbit anti-dopamine D3R receptor (MilliporeSigma), rabbit anti-dopamine D4R receptor (Invitrogen), and rabbit anti-dopamine D5R receptor (Invitrogen).

    Techniques: Control, XTT Assay

    Information on primary antibodies.

    Journal: Frontiers in Neuroanatomy

    Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

    doi: 10.3389/fnana.2023.1111008

    Figure Lengend Snippet: Information on primary antibodies.

    Article Snippet: Dopamine receptor D2 (D2R) , Cytoplasmic loop #3 (28 aa) of human D2R , Millipore; rabbit polyclonal; Cat# AB5084P, RRID:AB_2094980 , 1:500.

    Techniques: Purification

    Distribution of each dopamine receptor in the motor cortex and caudate putamen. (A) Representative immunohistochemistry of the frontal cortex for D1R. High-magnification images of rectangles indicated with E and I are shown in (E,I) . Moderate to strong D1R-immunoreactivities were observed in the pial surface of the cerebral cortex, layer (VI), and caudate putamen. (B) Representative immunohistochemistry of the frontal cortex for D2R. High magnification images of rectangles indicated with F and J are shown in (F) and (J) . (C) Representative immunohistochemistry of the frontal cortex for D4R. High magnification images of rectangles indicated with G and K are shown in (G) and (K) . (D) Representative immunohistochemistry of the frontal cortex for D5R. High magnification images of rectangles indicated with H and L are shown in (H) and (L) . (E–H) Immunohistochemical images of the cortical surface for D1R (E) , D2R (F) , D4R (G) , and D5R (H) . (I) Immunohistochemical images of the cortical layer V for D1R. Neuronal somata show weak immunopositive signals for D1R (arrowheads). (J) Immunohistochemical images of the cortical layer V for D2R. Neuronal somata show weak immunoreactivity for D2R (arrowheads). (K,L) Immunohistochemical images of the cortical layer V for D4R or D5R. Many neuronal somata (arrowheads) and apical dendrites (arrows) show immunoreactivity for D4R or D5R. Scale bars: 500 μm (A–D) ; 125 μm (E–H) ; 50 μm (I–L) .

    Journal: Frontiers in Neuroanatomy

    Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

    doi: 10.3389/fnana.2023.1111008

    Figure Lengend Snippet: Distribution of each dopamine receptor in the motor cortex and caudate putamen. (A) Representative immunohistochemistry of the frontal cortex for D1R. High-magnification images of rectangles indicated with E and I are shown in (E,I) . Moderate to strong D1R-immunoreactivities were observed in the pial surface of the cerebral cortex, layer (VI), and caudate putamen. (B) Representative immunohistochemistry of the frontal cortex for D2R. High magnification images of rectangles indicated with F and J are shown in (F) and (J) . (C) Representative immunohistochemistry of the frontal cortex for D4R. High magnification images of rectangles indicated with G and K are shown in (G) and (K) . (D) Representative immunohistochemistry of the frontal cortex for D5R. High magnification images of rectangles indicated with H and L are shown in (H) and (L) . (E–H) Immunohistochemical images of the cortical surface for D1R (E) , D2R (F) , D4R (G) , and D5R (H) . (I) Immunohistochemical images of the cortical layer V for D1R. Neuronal somata show weak immunopositive signals for D1R (arrowheads). (J) Immunohistochemical images of the cortical layer V for D2R. Neuronal somata show weak immunoreactivity for D2R (arrowheads). (K,L) Immunohistochemical images of the cortical layer V for D4R or D5R. Many neuronal somata (arrowheads) and apical dendrites (arrows) show immunoreactivity for D4R or D5R. Scale bars: 500 μm (A–D) ; 125 μm (E–H) ; 50 μm (I–L) .

    Article Snippet: Dopamine receptor D2 (D2R) , Cytoplasmic loop #3 (28 aa) of human D2R , Millipore; rabbit polyclonal; Cat# AB5084P, RRID:AB_2094980 , 1:500.

    Techniques: Immunohistochemistry, Immunohistochemical staining

    D2R-immunoreactivity in astrocytes. (A,B) Very weak immunoreactivities for D2R were observed in the pial surface of the prelimbic (A) and somatosensory (B) areas. (C,D) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated a few weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the prelimbic area. (E,F) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the primary motor area. (G,H) Weak D2R-immunoreactivities were observed in layer I astrocytes (arrowheads) in the somatosensory area. (I,J) In layer III of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (arrowheads), but not in GS-positive protoplasmic astrocytes (arrows). (K,L) In layer V of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (asterisk), but only very weak in GS-positive protoplasmic astrocytes (arrows). Nuclei were stained with Hoechst33342. Scale bars: 20 μm (A,B) ; 5 μm (C,D) , 10 μm (E–L) .

    Journal: Frontiers in Neuroanatomy

    Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex

    doi: 10.3389/fnana.2023.1111008

    Figure Lengend Snippet: D2R-immunoreactivity in astrocytes. (A,B) Very weak immunoreactivities for D2R were observed in the pial surface of the prelimbic (A) and somatosensory (B) areas. (C,D) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated a few weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the prelimbic area. (E,F) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the primary motor area. (G,H) Weak D2R-immunoreactivities were observed in layer I astrocytes (arrowheads) in the somatosensory area. (I,J) In layer III of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (arrowheads), but not in GS-positive protoplasmic astrocytes (arrows). (K,L) In layer V of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (asterisk), but only very weak in GS-positive protoplasmic astrocytes (arrows). Nuclei were stained with Hoechst33342. Scale bars: 20 μm (A,B) ; 5 μm (C,D) , 10 μm (E–L) .

    Article Snippet: Dopamine receptor D2 (D2R) , Cytoplasmic loop #3 (28 aa) of human D2R , Millipore; rabbit polyclonal; Cat# AB5084P, RRID:AB_2094980 , 1:500.

    Techniques: Imaging, Double Immunostaining, Staining