dopamine receptor d2 (d2r) cytoplasmic loop #3 (28 aa) of human d2r (Millipore)
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Dopamine Receptor D2 (D2r) Cytoplasmic Loop #3 (28 Aa) Of Human D2r, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex"
Article Title: D1- and D2-type dopamine receptors are immunolocalized in pial and layer I astrocytes in the rat cerebral cortex
Journal: Frontiers in Neuroanatomy
doi: 10.3389/fnana.2023.1111008
Figure Legend Snippet: Information on primary antibodies.
Techniques Used: Purification
Figure Legend Snippet: Distribution of each dopamine receptor in the motor cortex and caudate putamen. (A) Representative immunohistochemistry of the frontal cortex for D1R. High-magnification images of rectangles indicated with E and I are shown in (E,I) . Moderate to strong D1R-immunoreactivities were observed in the pial surface of the cerebral cortex, layer (VI), and caudate putamen. (B) Representative immunohistochemistry of the frontal cortex for D2R. High magnification images of rectangles indicated with F and J are shown in (F) and (J) . (C) Representative immunohistochemistry of the frontal cortex for D4R. High magnification images of rectangles indicated with G and K are shown in (G) and (K) . (D) Representative immunohistochemistry of the frontal cortex for D5R. High magnification images of rectangles indicated with H and L are shown in (H) and (L) . (E–H) Immunohistochemical images of the cortical surface for D1R (E) , D2R (F) , D4R (G) , and D5R (H) . (I) Immunohistochemical images of the cortical layer V for D1R. Neuronal somata show weak immunopositive signals for D1R (arrowheads). (J) Immunohistochemical images of the cortical layer V for D2R. Neuronal somata show weak immunoreactivity for D2R (arrowheads). (K,L) Immunohistochemical images of the cortical layer V for D4R or D5R. Many neuronal somata (arrowheads) and apical dendrites (arrows) show immunoreactivity for D4R or D5R. Scale bars: 500 μm (A–D) ; 125 μm (E–H) ; 50 μm (I–L) .
Techniques Used: Immunohistochemistry, Immunohistochemical staining
Figure Legend Snippet: D2R-immunoreactivity in astrocytes. (A,B) Very weak immunoreactivities for D2R were observed in the pial surface of the prelimbic (A) and somatosensory (B) areas. (C,D) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated a few weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the prelimbic area. (E,F) Confocal imaging of double immunostaining for D2R (magenta) and GS (green) indicated weak D2R-immunoreactivities in pial astrocytes (arrowheads) in the primary motor area. (G,H) Weak D2R-immunoreactivities were observed in layer I astrocytes (arrowheads) in the somatosensory area. (I,J) In layer III of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (arrowheads), but not in GS-positive protoplasmic astrocytes (arrows). (K,L) In layer V of the primary motor area, D2R-immunoreactivities (magenta) were observed in GS-negative cells (asterisk), but only very weak in GS-positive protoplasmic astrocytes (arrows). Nuclei were stained with Hoechst33342. Scale bars: 20 μm (A,B) ; 5 μm (C,D) , 10 μm (E–L) .
Techniques Used: Imaging, Double Immunostaining, Staining
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